Coding

Part:BBa_K2906015:Design

Designed by: Sara ElSafadi, Ewan Egan   Group: iGEM19_Manchester   (2019-10-15)


COMT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 36
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 75
    Illegal AgeI site found at 258
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the gene is plant-derived, had to codon optimise it using GeneArt. His-Tag fused after cloning into pETDuet-1 plasmid.


Source

Sequence comes from Arabidopsis thaliana (UniProtKb P21964).

References